Technical Highlight - March 2010
Short description: A wiki containing all the procedures and experimental set-ups used by the Northeast Structural Genomics Consortium is ideal for training new spectrometrists.
The combined NMR spectroscopic wisdom of Gaetano Montelione, Cheryl Arrowsmith, Mark Girvin, Michael Kennedy, John Markley, Robert Powers, James Prestegard and Thomas Szyperski and their teams is now available to everyone in the NESG wiki.
Although the majority (roughly 90%) of the Protein Structure Initiative (PSI) structures have been solved by X-ray crystallography, NMR spectroscopy is a very important technique for structural genomics. This is partly because NMR can often succeed where numerous crystallographic attempts have failed, and partly because it provides information about how proteins behave in a dynamic environment.
While working on their pipeline, PSI NESG used an internal wiki to share the methods they were using. Over 50 subscribers have entered valuable information about protein sample production, NMR data acquisition, NMR data processing, NMR resonance assignment and structure calculation and validation. More than 150 pages are currently available, and this number is sure to grow now that it is open to the wider world.
This wiki isn't aimed at absolute beginners, as they assume that their target audience has some knowledge about NMR and protein structure determination. But the content would be very useful for training graduate students and postdocs, and even the most experienced spectrometrist is likely to find something useful here.