PSI Structural Biology Knowledgebase

PSI | Structural Biology Knowledgebase
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Cancer
Cell biology
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Drug discovery
Epigenetics
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Membrane proteins
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Protein design
Protein-protein interactions

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Target Selection
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E-Collection Research Themes: Membrane proteins

Related Articles

Drug Discovery: Solving the Structure of an Anti-hypertension Drug Target

Featured Article - July 2015
Serial femtosecond crystallography is used to solve the structure of angiotensin II type 1 receptor.

Retrospective: 7,000 Structures Closer to Understanding Biology

Featured Article - July 2015
As PSI:Biology comes to a close this month, we take a look back at all the biological advances made by the PSI Network.

Families in Gene Neighborhoods

Technical Highlight - June 2015
A bioinformatics strategy takes advantage of the proximal organization of genes encoding proteins involved in metabolic pathways to predict protein function.

Channels and Transporters: BEST in Show

Featured Article - April 2015
Crystal structures of bestrophin ion channels unravel the molecular basis of retinopathies.

Channels and Transporters: Reorienting a Peptide in the Pocket

Featured Article - April 2015
In POT transporters, peptide length determines whether the orientation of the ligand is lateral or vertical.

Ryanodine Receptor

Featured System - April 2015
PSI researchers take a close look at a gated calcium channel that controls muscle contraction.

Protein Folding and Misfolding: It's the Journey, Not the Destination

Technical Highlight - March 2015
Computational analysis suggests ∼10% of pathogenic mutations interfere with membrane integration.

Protein Folding and Misfolding: Refolding in Membrane Mimetic

Technical Highlight - March 2015
Using a membrane mimetic system, researchers produce properly folded integral membrane proteins that are suitable for subsequent X-ray crystallography analysis.

Nuclear Pore Complex: A Flexible Transporter

Featured Article - February 2015
Structural flexibility in HEAT motifs enables proteins to pass through the nuclear pore complex.

Nuclear Pore Complex: Higher Resolution of Macromolecules

Technical Highlight - February 2015
Integrating mass spectrometry data for crosslinked oligomers reveals details of the yeast nuclear pore complex.

Nuclear Pore Complex: Integrative Approach to Probe Nup133

Featured Article - February 2015
The integration of three structural methods offers insight into membrane association of the NPC.

Piecing Together the Nuclear Pore Complex

Featured System - February 2015
PSI researchers have solved another piece in the puzzle for revealing an atomic-level model of the nuclear pore complex.

Mitochondrion: Flipping for UCP2

Featured Article - December 2014
NMR studies reveal how UCP2 facilitates translocation of protons across the mitochondrial membrane via fatty acids.

Transmembrane Spans

Technical Highlight - December 2014
Analyses of nine topology prediction programs identify those with the greatest prediction accuracy for specific transmembrane transport protein families, and lead the authors to propose several distinguishable evolutionary pathways for these proteins.

Glucagon Receptor

Featured System - April 2014
PSI researchers have solved the structures of a GPCR that binds to peptide hormones, revealing new modes of GPCR recognition.

Membrane Proteome: A Cap on Transport

Featured Article - April 2014
Structural and biophysical analyses of a membrane carrier ectodomain reveal a Ca2+-dependent switch that regulates ATP flux across the mitochondrial inner membrane.

Membrane Proteome: Microcrystals Yield Big Data

Technical Highlight - April 2014
An X-ray free-electron laser helps determine the first room-temperature structure of a G protein-coupled receptor.

Membrane Proteome: Pumping Out Heavy Metal

Featured Article - April 2014
Crystal structures of a heavy-metal efflux pump reveal intermediate transport states.

Design and Discovery: Virtual Drug Screening

Technical Highlight - January 2014
Optimized crystal structure-based models yield diverse hits in a virtual screen targeting G protein-coupled receptor allosteric and orthosteric ligand-binding sites.

G Proteins and Cancer

Featured System - November 2013
G-proteins play an essential role in cell signaling, a role that is corrupted in many types of cancer cells.

Drug Discovery: Antidepressant Potential of 6-NQ SERT Inhibitors

Featured Article - October 2013
Molecular docking models are combined with in vitro and in vivo assays to evaluate the therapeutic potential of 6-nitroquipazine analogs as antidepressants.

Drug Discovery: Modeling NET Interactions

Featured Article - October 2013
A structural model of the norepinephrine transporter is used in a virtual ligand screen to identify new drug interactions.

Microbiome: Solid-State NMR, Crystallized

Technical Highlight - September 2013
The structure of a bacterial membrane protein was determined using solid-state NMR methods on protein microcrystals.

CAAX Endoproteases

Featured System - August 2013
Structures of two Ste24p-family proteases locate the active site inside a hollow, membrane-spanning chamber.

Membrane Proteome: A Funnel-like Viroporin

Featured Article - August 2013
The NMR structure of HCV p7 channel reveals a funnel-like architecture, providing insight into cation conductance and drug-mediated inhibition.

Membrane Proteome: GPCR Substrate Recognition and Functional Selectivity

Featured Article - August 2013
Crystal structures of 5-HT receptors bound to antimigraine drugs offer insights into ligand specificity and signaling bias.

Membrane Proteome: Making DNA Nanotubes for NMR Structure Determination

Technical Highlight - August 2013
A protocol for designing DNA nanotube liquid crystals to be used as a weak-alignment medium for high-resolution NMR is presented.

Membrane Proteome: Unveiling the Human α-helical Membrane Proteome

Technical Highlight - August 2013
All PSI Membrane Protein Structure Centers and one large-scale production center have joined forces to create a strategy to maximize our understanding of the human transmembrane proteome.

Cell-Cell Interaction: Magic Structure from Microcrystals

Technical Highlight - March 2013
Structure determination of a membrane protein using poorly diffracting microcrystals illustrates a powerful new application of solid-state NMR spectroscopy.

Cell-Cell Interaction: Nanoparticles in Cell Camouflage

Technical Highlight - March 2013
Membranes of white blood cells are harnessed to improve drug-delivery vehicles.

Membrane Proteome: Capturing Multiple Conformations

Featured Article - December 2012
Four novel crystallized conformers of the betaine/Na+ symporter BetP suggest a structural mechanism for molecular transport.

Membrane Proteome: Soft Sampling

Technical Highlight - December 2012
A novel method for processing non-uniformly sampled NMR data is orders of magnitude faster and permits very sparse sampling for challenging applications.

Membrane Proteome: Sphingolipid Synthesis Selectivity

Featured Article - December 2012
Substrate specificity in trypanosomal sphingolipid synthases can be determined by variation in as few as three residues.

Membrane Proteome: Tuning Membrane Protein Expression

Technical Highlight - December 2012
A tunable E. coli strain results in less protein aggregation in vivo and more functional, membrane-inserted protein.

Cytochrome Oxidase

Featured System - November 2012
Cytochrome oxidase is the foundation of aerobic respiration.

Membrane Proteome: Building a Carrier

Featured Article - November 2012
NMR methods and molecular fragment searching are used to generate a structural model of a mitochondrial carrier protein.

Membrane Proteome: Every Protein Has Its Tag

Technical Highlight - November 2012
A tag variation strategy enables increased membrane protein production.

Membrane Proteome: Specific vs. Non-specific weak interactions

Technical Highlight - November 2012
Using a series of NMR experiments, weak interactions between membrane proteins can be classified as specific or nonspecific.

Membrane Proteome: The ABCs of Transport

Featured Article - November 2012
Structural analyses of two ABC transporters reveal how diverse transmembrane domain architectures determine substrate specificity and transport mechanisms.

Bacterial Phosphotransferase System

Featured System - October 2012
Bacteria are thrifty cells.

Insert Here

Technical Highlight - October 2012
A method to identify fusion partners for GPCRs can lead to new structures.

Solute Channels

Featured System - September 2012
Cells maintain a steady traffic of small molecules across their membranes.

To structure, faster

Technical Highlight - August 2012
Backbone structure determination of human integral membrane proteins is enabled by cell-free protein expression followed by solution NMR spectroscopy.

Pocket changes

Technical Highlight - July 2012
Pocketome, an encyclopedia of small-molecule binding sites, helps analyze druggable sites.

Predictive protein origami

Technical Highlight - July 2012
Membrane protein 3D folds are accurately predicted from evolutionary constraints derived from genomic sequencing.

G Protein-Coupled Receptors

Featured System - May 2012
In the past five years, the field of GPCR structure has exploded.

Twist to open

Featured Article - March 2012
A structure of an engineered potassium channel suggests a novel model for the gating mechanism.

Anchoring's the way

Featured Article - February 2012
New insights into the regulation of AQP0 reveal mechanisms involved in cataract development.

Overexpressed problems

Technical Highlight - February 2012
An analysis of the effects of protein overexpression gives insight into the difficulties of preparing intramembrane proteins for crystallization.

Gentle membrane protein extraction

Technical Highlight - January 2012
Noncovalent chelation of nickel-doped PEG polymers to His-tagged integral membrane proteins permits the solubilization of functional proteins in low-detergent or detergent-free aqueous buffer.

Docking and rolling

Featured Article - October 2011
GPCR Dock 2010 reveals just how far homology modeling has come and how far it still has to go.

A fragmented approach to membrane protein structures

Technical Highlight - September 2011
An innovative method combines NMR experiments and a database search to determine the structure of a challenging membrane protein.

Raising a glass to GLIC

Featured Article - August 2011
The pentameric ligand-gated ion channels (pLGICs) are known to be affected by alcohol in eukaryotes. Analyses of the prokaryotic GLIC suggest that it may be a good structural model for the effects of n-alcohols on pLGICs.

Sugar transport

Featured Article - June 2011
The structure of an EIIC from Bacillus cereus shows the architecture of this important class of sugar transporters.

A2A Adenosine Receptor

Featured System - May 2011
With proteins, small motions often have large effects.

TrkH Potassium Ion Transporter

Featured System - April 2011
Cells are filled with a salty soup of metal ions.

Subtly different

Featured Article - March 2011
The crystal structure of the D3 dopamine receptor reveals how to selectively target D2-like receptors.

A new amphiphile for crystallizing membrane proteins

Technical Highlight - January 2011
An easy-to-use chemical approach for stabilizing, extracting and crystallizing integral membrane proteins.

CXCR4

Featured System - January 2011
The GPCR (G protein-coupled receptor) community is suffering from an embarrassment of riches.

Guard cells pick up the SLAC

Featured Article - December 2010
Guard cells rely on anion channels to regulate stomata in response to environmental cues. A new crystal structure gives insight into how stomata close.

ABA receptor diversity

Featured Article - November 2010
There are 14 members in the ABA receptor family in Arabidopsis thaliana. How these receptors differ in their response to a synthetic, seed-specific ABA agonist is now explored in two independent papers, using a combination of crystallography, NMR and biochemical approaches.

COX inhibition: Naproxen by proxy

Featured Article - November 2010
Structural basis of COX inhibition suggests that adverse effects of NSAIDs will not be easily avoided.

Zinc Transporter ZntB

Featured System - July 2010
Zinc is an essential component of many cellular processes.

Formate transporter or channel?

Featured Article - March 2010
The mystery of how formate crosses the membrane is now solved.

Tips for crystallizing membrane proteins in lipidic mesophases

Technical Highlight - February 2010
Advice on this useful technique for crystallizing integral membrane proteins.

Urea transporter

Featured Article - February 2010
The X-ray crystal structure of a bacterial homolog of the mammalian urea transporter reveals a highly specific, channel-like transport mechanism.

Five good reasons to use single protein production for membrane proteins

Technical Highlight - January 2010
A refinement of the SPP method eliminates lengthy purification steps in the pursuit of membrane proteins suitable for structural studies.

Membrane proteins spotted in their native habitat

Featured Article - January 2010
A modest reshaping of the lipid bilayer allows water molecules to hydrate voltage-gating channels and focus the electrical field.

Spot the pore

Technical Highlight - January 2010
Straightforward web-based software pinpoints the central channel of any transmembrane protein.

Get3 into the groove

Featured Article - October 2009
Unlike most membrane proteins, SNAREs enter the endoplasmic reticulum after translation. The structure of the ATPase needed for entry reveals the likely mechanism.

GPCR subunits: Separate but not equal

Featured Article - September 2009
A functional complementation assay reveals that maximal heterotrimeric G-protein activation is achieved by agonist binding to one subunit of a dopamine D2 receptor dimer.

GPCR modeling: any good?

Technical Highlight - August 2009
Results from a competition that invited groups to predict the structure of a GPCR show some promise, but accurate modeling of these receptors remains a challenge.Nature Rev. Drug Discov. 8, 455-463 (2009)

Surviving in an acid environment

Featured Article - August 2009
The Escherichia coli O157:H7 amino acid antiporter structure reveals clues to how this bacterium survives in the harsh stomach environment.Science 324, 1565-1568 (2009)

Tips for crystallizing membrane proteins

Technical Highlight - June 2009
A practical guide to cloning, expressing, purifying and crystallizing membrane proteins.Nat. Protoc. 4, 619-637 (2009)

You look familiar: the Type VI secretion system

Featured Article - June 2009
New structures raise the perplexing question of which came first, the bacterial secretion system or the bacteriophage tail.Proc. Natl Acad. Sci. USA 106, 4154-4159 (2009)

Bacterial Leucine Transporter, LeuT

Featured System - May 2009
Many antidepressant drugs, as well as drugs of abuse such as cocaine, block the traffic of neurotransmitters across the cell membrane of nerve axons and glial cells.

Aquaglyceroporin

Featured System - March 2009
The protozoan parasites that cause malaria multiply rapidly in the blood of infected individuals.

Death clusters

Featured Article - March 2009
The structure of the Fas–FADD death domain complex reveals how it switches on apoptosis solely through oligomerization and clustering.Nature, doi: 10.1038/nature07606

Protein nanopores

Technical Highlight - March 2009
Ultra high-speed DNA sequencing could be a step nearer as the protein channel MpsA is found to be an excellent nanopore.Proc. Natl. Acad. Sci. USA 105, 20647-20652 (2008)

Transporter mechanism in sight

Featured Article - February 2009
Structural studies of the leucine transporter reveal details of the early steps in substrate transport across the membrane.Science 322, 1655-1661 (2008)

A pocket guide to GPCRs

Featured Article - December 2008
The structure of the G-protein-coupled receptor adenosine receptor A2A reveals differences between its ligand-binding site and those of other family members, and begins to explain this family's diversity.Science, doi:10.1126/science.1164772

Tuning membrane protein overexpression

Technical Highlight - October 2008
Proc. Natl Acad. Sci. USA 105, 14371-14376 (2008)

Blocking AmtB

Featured Article - September 2008
Proc Natl Acad Sci USA 104, 42-47 (2007)
Structural Biology Knowledgebase ISSN: 1758-1338
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