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id="title" class="nomar"Membrane Protein Structural Biology Consortium

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The MPSBC is a PSI Membrane Protein Structure Determination Center, and consists of investigators from the Hauptman-Woodward Institute, The University of Virginia, and the University of Rochester.

Contact Information

Michael Malkowski

Mark Dumont

Michael Wiener

Research Description

The MPSBC brings the diverse expertise of three investigators to bear on central problems of membrane protein structural biology, with emphasis upon overcoming the technical barriers, using improved technologies to solve structures, and disseminating these improvements to the biological and structural biological communities. The involvement of the three different laboratories also brings together a range of pre-existing facilities and personnel, ongoing structural projects, ties to collaborators working on important targets, and existing infrastructures for interacting with the broader scientific community.

Key Publications
  1. Koszelak-Rosenblum et al. Determination and application of empirically derived detergent phase boundaries to effectively crystallize membrane proteins.Protein Sci. (2009) Sep; 18(9):1828-39. (PubMed ID: 19554626).

  2. Clark et al. Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography. Protein Expr Purif. (2010) Jun; 71(2):207-23. (PubMed ID: 20045057).

  3. Malkowski et al. Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing. Proc Natl Acad Sci U S A. (2007) Apr 17;104(16):6678-83 (PubMed ID: 17426150).

  4. Vergis et al. A High-Throughput Differential Filtration Assay to Screen and Select Detergents for Membrane Proteins. (PubMed ID: 20667442).

Structures, Targets, Publications and Technologies

MPSBC Structures in RCSB PDB
MPSBC Targets in TargetTrack
MPSBC SBKB Publications Page
MPSBC SBKB Technology Portal Page

Relevant Links

MPSBC homepage
Center for High-Throughput Structural Biology (PSI-2 center)

Structural Biology Knowledgebase ISSN: 1758-1338
Funded by a grant from the National Institute of General Medical Sciences of the National Institutes of Health