PSI Structural Biology Knowledgebase

PSI | Structural Biology Knowledgebase
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id="title" class="nomar"Partnership for T-Cell Biology

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This biological project consists of investigators from The Scripps Research Institute (TSRI), and is partnered with the PSI High-Throughtput Structure Determination center JCSG.

Member Institutions

  • The Scripps Research Institute

Contact Information

James R. Williamson
jrwill@scripps.edu

Daniel R. Salomon
dsalomon@scripps.edu

Research Description

Our work combines genomic data analysis, biochemistry, structural biology, and functional characterization to understand the role of ribonucleoprotein complexes in regulating T-cell activation. Expression data were mined to identify a set of RNA binding proteins that were either differentially expressed or alternatively spliced, upon T-cell activation. These RNA binding proteins constitute the input into a structural genomics effort, representing the first of three structural target streams. Protein interacting partners for these RNA binding proteins have been identified from the Human Interactome Database, and the formation of specific protein-protein complexes will be confirmed both in T-cells and in vitro. The resulting set of protein-protein complexes will be input into a structural genomics effort as the second target stream. The RNA targets for the RNA binding proteins will be identified using high throughput sequencing and crosslinking (HITS-CLIP), and confirmed in T-cells and in vitro. The resulting set of RNA-protein complexes will be input into a structural genomics effort as the third target stream. For the proteins, protein-protein complexes, and RNA-protein complexes that emerge from the structural genomics effort, functional validation will be carried out in T-cells to determine the role of these specific components in T-cell activation. This work should provide significant new insights into the structure of ribonucleoprotein complexes in general, as well as insights into how these complexes are involved in posttranscriptional gene regulation.

Structures, Targets, Publications and Technologies

TCELL Structures in RCSB PDB
TCELL Targets in TargetTrack

Relevant Links

Williamson Lab Website
Salomon Lab Website

Structural Biology Knowledgebase ISSN: 1758-1338
Funded by a grant from the National Institute of General Medical Sciences of the National Institutes of Health