id="title" class="nomar"Transcontinental EM Initiative for Membrane Protein Structure
The TEMIMPS center consists of investigators from 6 institutions: Skirball Institute - New York University Medical Center, Mount Sinai School of Medicine, Case Western Reserve University, University of Washington, The University of Texas - Houston Medical School, and New York Structural Biology Center.
- Skirball Institute - New York University Medical Center
- Mount Sinai School of Medicine
- Case Western Reserve University
- University of Washington
- The University of Texas - Houston Medical School
- New York Structural Biology Center
The Transcontinental EM Initiative for Membrane Protein Structure (TEMIMPS) is a research consortium funded by the NIH Protein Structure Initiative:Biology program. At TEMIMPS, we are dedicated to structure determination of membrane proteins by cryo-electron microscopy (cryo-EM). This approach is well established for producing intermediate and atomic resolution structures and offers the following advantages: (1) Membrane proteins can be studied within their native lipid environment. (2) Reduced steric constraints, either within membrane crystals or in isolated, detergent-solubilized complexes, facilitate interactions between membrane proteins and their binding partners, such as ligands and effector proteins. (3) A three-dimensional map can be produced with poorly ordered, or non-crystalline material, thus providing a template for molecular modeling and characterization of conformational change. In particular, we are developing a pipeline that includes two-dimensional crystallization of membrane proteins, electron cryo-microscopy and image analysis to achieve this goal. Six different laboratories from across the United States participate in TEMIMPS. Investigators from these laboratories have established expertise in cryo-electron microscopy, electron crystallography and membrane proteins.