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id="title" class="nomar"Transcontinental EM Initiative for Membrane Protein Structure

The TEMIMPS center consists of investigators from 6 institutions: Skirball Institute - New York University Medical Center, Mount Sinai School of Medicine, Case Western Reserve University, University of Washington, The University of Texas - Houston Medical School, and New York Structural Biology Center.

TEMIMPS investigators

  • Skirball Institute - New York University Medical Center
    David Stokes
  • Mount Sinai School of Medicine
    Iban Ubarretxena-Belandia
  • Case Western Reserve University
    Andreas Engel
  • University of Washington
    Tamir Gonen
  • The University of Texas - Houston Medical School
    Pawel Penczek
  • New York Structural Biology Center
    James Love

Contact Information

David Stokes
stokes@nyu.edu

Iban Ubarretxena-Belandia
Iban.Ubarretxena@mssm.edu

Andreas Engel
ahe13@case.edu

Tamir Gonen
tgonen@u.washington.edu

Pawel Penczek
Pawel.A.Penczek@uth.tmc.edu

James Love
jlove@nysbc.org

 Research Description

The Transcontinental EM Initiative for Membrane Protein Structure (TEMIMPS) is a research consortium funded by the NIH Protein Structure Initiative:Biology program. At TEMIMPS, we are dedicated to structure determination of membrane proteins by cryo-electron microscopy (cryo-EM). This approach is well established for producing intermediate and atomic resolution structures and offers the following advantages: (1) Membrane proteins can be studied within their native lipid environment. (2) Reduced steric constraints, either within membrane crystals or in isolated, detergent-solubilized complexes, facilitate interactions between membrane proteins and their binding partners, such as ligands and effector proteins. (3) A three-dimensional map can be produced with poorly ordered, or non-crystalline material, thus providing a template for molecular modeling and characterization of conformational change. In particular, we are developing a pipeline that includes two-dimensional crystallization of membrane proteins, electron cryo-microscopy and image analysis to achieve this goal. Six different laboratories from across the United States participate in TEMIMPS. Investigators from these laboratories have established expertise in cryo-electron microscopy, electron crystallography and membrane proteins.

 

Key Publications
  1. Kim C, Vink M, Hu M, Love J, Stokes DL, Ubarretxena-Belandia I. 2010
    An automated pipeline to screen membrane protein 2D crystallization.

    J Struct Funct Genomics,11:155-66 (PubMed ID: 20349145).
  2. Hu M, Vink M, Kim C, Derr K, Koss J, D'Amico K, Cheng A, Pulokas J, Ubarretxena-Belandia I, Stokes D. 2010
    Automated electron microscopy for evaluating two-dimensional crystallization of membrane proteins.

    J. Struct. Biol.,171:102-110 (PubMed ID: 20197095).
  3. Coudray N, Hermann G, Caujolle-Bert D, Karathanou A, Erne-Brand F, Buessler JL, Daum P, Plitzko J, Chami M, Mueller U, Kihl H, Urban JP, Engel A, Remigy HW. 2010.
    Automated screening of 2D crystallization trials using transmission electron microscopy: A high-throughput tool-chain for sample preparation and microscopic analysis.

    J. Struct. Biol. in press. (PubMed ID: 20868753).
  4. Iacovache I, Biasini M, Kowal J, Kukulski W, Chami M, van der Goot FG, Engel A, Rémigy HW. 2010
    The 2DX robot: a membrane protein 2D crystallization Swiss Army knife.

    J. Struct. Biol.,169:370-8 (PubMed ID: 19963066).

Structures, Targets, Publications and Technologies

TEMIMPS Structures in RCSB PDB
TEMIMPS Targets in TargetTrack
TEMIMPS SBKB Publications Page
TEMIMPS SBKB Technology Portal Page

Relevant Links

TEMIMPS homepage

Structural Biology Knowledgebase ISSN: 1758-1338
Funded by a grant from the National Institute of General Medical Sciences of the National Institutes of Health