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id="title" class="nomar"Transmembrane Protein Center

First Row (L to R): Marley Crews-Hill, Trang Nguyen, Rachel Schwanz, Andrew Gesior, Nicole Reinen, Fabian Suchy, Jakki Saunders. Second Row: Leah Schmitz, Jon Stefley, Kylie Moyer, Emily Beebe, Tina Misenheimer, Hannah Meddaugh. Third Row: John Markley (MPP PI), John Primm (Project Manager), Brian Fox (TMPC PI), Shin-ichi Makino, Otto Kletzien. Back Row: David Pagliarini (MPP PI), Ronnie Frederick, Andrew Reidenbach, Russell Wrobel, Dave Aceti, Don Drott, Jameson Bothe. (Not Pictured: Kasia Gromek and Donna Troestler)

The TMPC is a PSI Membrane Protein Structure Determination Center located at the University of Wisconsin-Madison. It is partnered with the PSI High-Throughput Structure Determination Center MCSG.

TMPC Investigators

  • University of Wisconsin-Madison
    Brian Fox, PI
    John Markley
    George Phillips, Jr.

Contact Information

Brian Fox, PI
bgfox@biochem.wisc.edu

John Primm, Project Manager
jgprimm@wisc.edu

Research Description

The University of Wisconsin Transmembrane Protein Center (TMPC) will function as a collaborative project within the PSI:Biology Network. The collective experience of our already-assembled research team suggests that application of many techniques will be needed to expand the chances for success with the broad range of membrane proteins expected from participation in the PSI:Biology Network. This project is well poised to provide the expertise and leadership needed to successfully advance the goals of the PSI:Biology Network, including outreach to new participants in this large-scale endeavor. We have experience in the expression, production, and characterization of membrane proteins, proven ability to solve structures of complicated membrane proteins, and a strong track record of studying the interactions of membrane proteins with their protein partners and biological ligands. We also have strong grounding in the use of bioinformatics and bioanalytical techniques. Every participant brings unique experience with the function of membrane proteins, including assay development, preparation methods, or studies with either purified proteins and living organisms. This emphasis on merging the production of membrane proteins for structure determination with the power of functional characterizations is the central philosophy of TMPC. Our primary goal is determining a founding structure for unique target classes. Our second goal is to establish an efficient membrane protein production pipeline so that increases in throughput may be progressively obtained. We expect to improve our ability to create protein variants, to refine screening based on functional and biophysical characterizations, and to improve sorting between multiple expression pathways to find the best way produce a protein of interest. Emphasis on small-scale purification screening, and further integration of automation into our efforts will increase efficiency. Expanded options for crystallization screening, access to synchrotrons, and improvements in software used to solve structures will also contribute to the increased throughput necessary to achieve PSI:Biology goals.

Mentions at PSI Structural Biology Knowledgebase

Cloning Techniques: PSI Centers Highlight their Cloning Successes
Function Following Form
Membrane Proteome: Sphingolipid Synthesis Selectivity
Membrane Protein Structures Enter an Exponential Growth Phase
Solve Your Solubility Problems: Wheat Germ Cell-Free Extract Might be the Answer to Protein Production Difficulties

Relevant Links

Transmembrane Protein Center
Center for Eukaryotic Structural Genomics (PSI-2)

Structural Biology Knowledgebase ISSN: 1758-1338
Funded by a grant from the National Institute of General Medical Sciences of the National Institutes of Health